Narrated video has annotated animations and live cell videos.
1. Database of mouse models of human cancers.
2. Information pertaining to research and techniques used for transgenic mice.
http://members.cox.net/microinjectionworkshop/
3. Online book on mouse genetics by Lee M. Silver.
http://www.informatics.jax.org/silver/
4. Trans-NIH Knockout Mouse Project (KOMP) - an initiative to generate a public resource of mouse embryonic stem (ES) cells containing a null mutation in every gene in the mouse genome.
5. Databases of electronic resources.
http://www.healthsystem.virginia.edu/internet/transgenic-mouse/links.cfm
Mouse (Mus musculus)
Lecture videos are available for viewing in the left column.May 28 class:
The first two videos on the left are part of a single HHMI movie.
1. Before class - please view video 1. Only minutes 3:00-5:00 about generating a transgenic mouse will be shown in class.
2. During class - video 2 will be used to illustrate key points about generating knock out and knock in mice.
June 4 class:
Videos will help you visualize the process of angiogenesis, please watch before class.
Required/Encouraged Reading
May 28 class:
1. In this review the Nobel laureate gives an wonderful historical overview of gene targeting in the mouse.
"Generating mice with targeted mutations" by Mario R. Capecchi
2. This review provides a comprehensive view of the mouse as a model organism, covering history, techniques and screens.
June 4 class:
Articles about the role of angiogenic factors in a mouse models of breast cancer.
1. Deletion of vascular endothelial growth factor in myeloid cells accelerates tumorigenesis by Stockmann C et al.
2. VEGF Restores Delayed Tumor Progression in Tumors Depleted of Macrophages by Lin EY et al.
3. A review providing information that will help you understand details in the above articles.
Monocyte/macrophage infiltration in tumors: modulators of angiogenesis by Dirkx AEM et al.
Additional Reading
May 28 class:
Review of approaches in mouse genetics - Detailed but easy to understand.
"Tools for targeted manipulation of the mouse genome" by Louise Van Der Weyden et al.
June 4 class:
1. Recent review discussing targeting of angiogenesis process in cancer.
Molecular Mechanisms and Therapeutic Development of Angiogenesis Inhibitors by Yihai Caoa.
2. General Angiogenesis overview and discussion of its role in cancer and other diseases.
Modulating angiogenesis: more vs less. by Sivakumar B, Harry LE and Paleolog EM.