Visualizing apoptosis with some very good 3-D animation by Drew Berry.
Molecular mechanisms underlying apoptosis in cell culture
Links to many worm movies are found on this web page.
Scroll down to the bottom of the 'embryo' section to see cell death during development of wild type and mutant worms.
The class will go with Giana to the Van Gilst lab after the lecture from ~3-3.30pm. We will get to see/do the following:
1. Wild type worms.
2. Mutant worms (they move really strangely! This is used as a marker as we discussed in class).
3. Live worms moving about giving off green fluorescence due to expression of GFP.
4. Pick up worms - Giana says brave souls can try picking up worms and setting up a cross if they want to - cool! I want to try this!
Overall, we will get a sense of the entire worm lab set up.
Roundworm (Caenorhabditis elegans)
Lecture videos are available for viewing in the left column. They will be used to illustrate points about the following:
Required/Encouraged Reading
1. A review about how the worm was developed as a model organism
"The natural history of Caenorhabditis elegans research" by Ankeny RA
2. A very compact apoptosis overview. It is a bit old but covers the core concepts.
"Advances in apoptosis research" by Peter ME, Heufelder AE, and Hengartner MO
3. A review on how the worm can contribute to cancer gene discovery.
FHCRC
scientists working with the worm model system:
1. Linda Buck: neurophysiology; drug screens affecting lifespan
2.
Jim Priess: developmental biology
3.
Mark Roth: suspended animation and "metabolic hibernation"
4. Marc Van Gilst: nutrient sensing and metabolic adaptation
These are excellent resources for C.elegans information. They provide very good information that is easily accessible.
1. A great starting point for all questions about C.elegans.
2. An overview of worm anatomy including a huge catalog of worm images (both real and cartoon), along with a basic descriptive overview of the tissue, organ or cell of interest.
3. The worm community's go-to place for news, links to worm resources, and information about any C.elegans gene of interest.
4. An educational portal dedicated to C.elegans.
If you're off-campus click on the 'FHCRC student' link in this box before trying to access full text course readings.
Once you've logged in, use your "back" button to return to this page and click on the links to the readings.
FHCRC students (sign in with your HutchNetID and password)