In DNA Lab 1, students become proficient in using gel electrophoresis while connecting concepts about DNA, enzymes, and charge. In this lab, students will run gel electrophoresis on precut lambda DNA and observe the differing banding patterns. Using a ladder, they can determine the approximate size of each fragment.
NEB's video on Type II Restriction Enzymes
Yes, Gel Kit crates can be a pain in the . . . But! There's hope. Here are three guides, one for each crate. If items just aren't behaving, it's better to put them in a separate container (box, grocery bag) and bring them in that way. We would rather have them returned separate and intact, rather than together, smooshed and broken.
A significant reduction in gel electrophoresis running time can be achieved by decreasing the concentration of the running buffer (TAE) and increasing the voltage:
BE SURE YOU:
If you exceed 20 minutes the gel will melt.
Under these conditions, the top edge of the gel (where the wells are located) may begin to melt a bit, but this should not impact the visualization of DNA fragments.
- Tip courtesy of Essy Levy of Bio-Rad