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SEPGuides: Science Education Partnership: ELISA Kit

SEPGuides is the go-to place for all SEP kit protocols and activities, as well as the posting area for announcements and program updates. If something is missing or you have an idea to contribute, please e-mail us at

2017 Updates

Disclaimer: These updates are still in progress. If you decide to use these with your class please give us feedback on how we could improve by emailing us at

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In The News

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How Immunofluorescence Works

Kit Description

3 Kits
2 Crates per Kit

ELISA logo
This kit is a simulated detection of avian flu influenza via an ELISA assay. Students are County Health Officials and are assigned the task of analyzing sera of a bird and a cat via ELISA to detect the presence of avian influenza. The kit also includes some models for demonstrating how the ELISA assay works and about antibody structure. Additional resources and journals are included to inform and educate students on the impending avian flu situation across the globe.

1) Teachers must attend a training workshop to sign up for an ELISA Kit.
2) Teacher supplies distilled water to dilute PBS, paper towels and timers for wash steps.


ELISA Notebook Table of Contents

Table of Contents  
Tab Document
Kit Inventory Complete List of Equipment and Materials Included
ELISA Kit Inventory List
Curriculum Overview Title Page
This is the Title Page for the ELISA Notebook.
  Enduring Understanding and Objectives
Key attitudes, knowledge and skills students will acquire as a result of this unit.
  Curriculum Flowchart
A summary of the Avian Flu curriculum and major topics.
  Curriculum Timeline
Lesson timeline based on 55-minute class periods.
  Activity Matrix
Adapted from the BSCS "5E Model".
  Science Standards
Topics the ELISA Kit covers in the National Science Standards.
  To the Teacher: Beginning Assumptions
Curriculum implementation suggestions.
Scenario Avian Flu Scenario
The initial scenario, introducing students to Garfield, the pigeon, and their research task.
  KWL Teaching Strategy
A link to the Indiana University's KWL Instruction webpage.
Avian Flu Bird Flu Facts
Background information on the H5N1 virus - designed for teachers but appropriate for advanced students.
  Epidemiology Map Activity
Using WHO data, students plot locations of human and animal H5N1 cases worldwide over time.
  Map Activity Overhead
Overhead transparency for the Map Activity.
  World Map
A 17in x 8.5in world map students can use to plot flyways and H5N1 outbreaks.
  WHO H5N1 Timeline
Link the the WHO webpage containing a PDF of H5N1 outbreaks catalogued by year.
  Google Earth
A tutorial on how to download and use the Google Earth mashup tracking H5N1, created by
Flu Virus and Antibodies Flu Virus: Infection of Cell Overhead and Separate Key
A 2-page graphic showing the various stages in the viral infection of a cell, along with a key of symbols and terminology.
  Flu Virus: Infection of Cell Overhead with Key
A 1-page graphic of viral infection of a cell and key.
  Avian Flu Terminology
This link will take you to the Clean Harbors website, which has created a useful dictionary of influenza terminology.
  The Double Helix and Immunology
This link will take you to the Nature article, covering the immune system, mechanisms of DNA reshuffling, and antibody formation.
  Modeling Antibodies with Chenille Stems Activity
An activity designed to teach students about the formation of antibodies by building models out of chenille stems.
  The Biology Project: Antibody Structure
This link will take you to the University of Arizona's website "The Biology Project", an interactive resource for learning biology. They have created a unit on anitibody structure that is very useful.
  SEP PyMOL Guide for the Protein Data Bank Activity
This guide is designed to help you locate any protein structure of interest and download it from the Protein Data Bank.
  PDB Molecule of the Month: Antibodies
Antibodies are the RCSB PDB Molecule of the Month for September 2001.
  PDB Molecule of the Month: Hemagglutinin
Hemagglutinin is the RCSB PDB Molecule of the Month for April 2006.
ELISA Planter Tray Model
Modeling the concepts utilized by the ELISA assay.
  ELISA - Teacher's Guide and Prep
Explanation of protocol, dilution procedures, and other information for the teacher to prepare reagents and materials for the ELISA Lab.
  Pre-lab Questions - Answer Key
Teacher's guide to pre-lab questions given to students.
  Usage Guide for 96-well Plates
An overhead describing how students should label and use the 96-well plates in the assay.
  ELISA Protocol - Student Version
The student protocol for the ELISA lab.
  ELISA Worksheet - Blank
A blank worksheet to be filled out by the students describing their results.
  ELISA Positive and Negative Control Overhead
An overhead teacher's can use to walk students through the ELISA process.
  ELISA Worksheet - Teacher's Key
A key for the expected results of the ELISA lab.
  ELISA Rubric - Letter From County Health Dept.
Expectations of students' understandings based on their final results and a written letter to a "County Health Department".
Pipetting Teacher's Guide: Measure for Measure
  Student Protocol: Measure for Measure
  Gilson-Rainin Protocol Card
Teacher Resources Example of Bird Flu Curriculum Submitted by Sue Black, Inglemoor HS
  Bird Flu Map Makeup Assaignment Submitted by Sue Black, Inglemoor HS

Additional Web Resources - Avian Flu

These are links to several websites used to develop the ELISA Kit and are excellent resources for additional information on current H5N1 research.

Additional Web Resources - Swine Flu

These are several links to Avian Flu's "cousin", Swine Flu, which is also a current source of concern for epidemiologists. Swine Flu is caused by the H1N1 virus.


H1N1 virus

H1N1 "swine flu" virus

Journal Articles

Links to articles used in the development of the ELISA Kit and important background information for teachers and advanced students.