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SEPGuides: Science Education Partnership: Gel Electrophoresis Kit
SEPGuides is the go-to place for all SEP kit protocols and activities, as well as the posting area for announcements and program updates. If something is missing or you have an idea to contribute, please e-mail us at email@example.com
Yes, Gel Kit crates can be a pain in the . . . But! There's hope. Here are three guides, one for each crate. If items just aren't behaving, it's better to put them in a separate container (box, grocery bag) and bring them in that way. We would rather have them returned separate and intact, rather than together, smooshed and broken.
A news article from Nature.com about genetic research. My favorite line: "Gene is not a typical four-letter word."
Colors by Guineau Delamare; Bernard Francois
Publication Date: 2000-11-01
Surveys the history of dyes & pigments, from the painted caves at Lascaux to today's computerized chemistry. (Discoveries Series).
The Color Revolution by Regina Lee Blaszczyk
Publication Date: 2012-08-31
The Color Revolution unites the visible history of the color chart with the hidden history of imperfect information, mood, and perception. The narrative arc of this beautifully illustrated book starts with the technical achievement of color in describing the timely development of a reproducible color system among specialists who became 'colorists.' An insightful must-have for the student and historian of business enterprise, industrial psychology, advertising, and the predictive modeling of nuance and effect.
This slideshow is a step-by-step walkthrough of how to make a DNA gel, using pictures for visual learners.
Gel electrophoresis is a commonly use tool for separating macromolecules (DNA, RNA, proteins) and their fragments based on size, shape, and charge. This kit allows students to use this technique to analyze the dyes used in sample loading buffers and DNA fragments.
SEP has 12 Gel Electrophoresis Kits and 2 Equipment-only Kits
Each Gel Kit has 3 crates.
Don't forget your Freezer Box!
Labs are designed for classes of 32 students. (Make sure to tell us if your class is significantly different in size.)
The Gel Kits can support any of these labs.
Measure for Measure
DNA Lab 1: Precut DNA
DNA Lab 1 (forensics)
DNA Lab 2: DNA Restriction Enzyme Digest
*Elephant Conservation (requires additional reservation of the Elephant Trunk)
A significant reduction in gel electrophoresis running time can be achieved by decreasing the concentration of the running buffer (TAE) and increasing the voltage:
Decrease the running buffer from 1X to 0.25X
Increase the voltage from 100V to 200V
Reduce your running time from 45 minutes to 20 minutes.
BE SURE YOU:
Make the agarose with 1X TAE NOT the 0.25X running buffer
Do NOT run the gel longer than 20 minutes.
If you exceed 20 minutes the gel will melt.
Under these conditions, the top edge of the gel (where the wells are located) may begin to melt a bit, but this should not impact the visualization of DNA fragments.
- Tip courtesy of Essy Levy of Bio-Rad
We recommend soaking the gel in 100 X Fast Blast for 3 minutes. Then rinse well (but carefully!) several times. It takes several rinses and some time to clear the background stain, but this process gives really great results. You can leave the gel destaining in a little water overnight and see really beautifully stained bands in the morning.