Table of Contents
Link to SEP's PCR Google Drive:
Introduction
PCR Labs
Additional Resources
DNA Ladders
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LMW DNA Ladder InfomationInformation and protocol for preparing NEB's Low Molecular Weight (LMW) Ladder. Includes a picture and bp size of the bands.
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100 bp DNA Ladder InformationInformation and protocol for NEB's Quick-Load 100 bp DNA Ladder. Note: DO NOT ADD STE or LOADING DYE.
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List of SEP PCR PrimersA list is the primer sequences we use for our PCR labs.
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Archive: OLD PCR Master Mix ProtocolArchive: Old PCR Master Mix Protocol for Teachers.
Virtual Labs and Games
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Virtual PCR Lab
PCR Virtual Lab by University of Utah
PCR Paper Model
Description
These activities are intended for biotech, genetics, AP, honors, or other advanced biology classes, and are available to SEP Teachers that have participated in SEP PCR workshops, or by permission. SEP supports these activities by supplying both the consumables and equipment (PCR machine, centrifuges, etc.). If you are interested is doing these activities with your class, contact SEP (sep@fredhutch.org, 206-667-4487).
PCR References
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The Unusual Origins of PCRMullis, Kary M., "The Unusual Origins of PCR". Scientific America. April 1990.
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Components of a PCR ReactionComponents and explanations of a PCR Reaction
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Animation of PCRAn animation of PCR by HHMI BioInteractive showing DNA during each step of the process.
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GeneAmp PCR Machine User GuideA quick user guide for SEP's GeneAmp PCR Machine.
PTC Taster PCR
PTC PCR Resources (updated 2017)
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PTC PCR Teacher BackgroundTeacher Background for PTC PCR outlining the science behind genotyping tasters using PCR and enzyme digest.
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PTC PCR Teacher GuideTeacher Guide for PTC PCR outlining the lab prep for each day, recipe guide for necessary reagents, and overview of the lab activity.
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PTC PCR Tech BackgroundStudent Background for PTC PCR outlining DNA extraction, PCR, enzyme digest, and gel electrophoresis.
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PTC PCR Tech GuideStudent Guide for PTC PCR outlining DNA extraction, PCR, enzyme digest, and gel electrophoresis.
Editable Resources
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PTC PCR Teacher Background (editable)Teacher Background for PTC PCR outlining the science behind genotyping tasters using PCR and enzyme digest.
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PTC PCR Teacher Guide (editable)Teacher Guide for PTC PCR outlining the lab prep for each day, recipe guide for necessary reagents, and overview of the lab activity.
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PTC PCR Tech Background (editable)Student Background for PTC PCR outlining DNA extraction, PCR, enzyme digest, and gel electrophoresis.
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PTC PCR Tech Guide (editable)Student Guide for PTC PCR outlining DNA extraction, PCR, enzyme digest, and gel electrophoresis.
Other Resources
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Taster SNP Introduction by Wellesley CollegeA thorough introduction to single nucleotide polymorphisms (SNPs) and a history and discovery of the PTC gene.
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LMW DNA Ladder InfomationInformation and protocol for preparing NEB's Low Molecular Weight (LMW) Ladder. Includes a picture and bp size of the bands.
GMO PCR
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GMO PCR Master Mix Recipe and PCR ProgramRecipe for making the GMO PCR Master Mix including temperatures and times for PCR.
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Isolation of DNA from FoodProtocol for isolating DNA from processed food using Chelex
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Strawberry DNA Isolation and PurificationTwo method for isolating DNA from strawberries for PCR.
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GMO PrimersLists the primer sets used for GMO PCR.
VNTR PCR (D1S80)
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D1S80 / ALU Protocol - Teacher's GuideTeacher's Guide for D1S80(VNTR) and PV92(ALU) PCR Protocols including master mix amounts and explanation of controls.
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PCR Machine Programing - D1S80/PV92/TAS2R38PCR machine programing temperatures for D1S80 (VNTR), PV92 (Alu), and TAS2R38 (TASTER).
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100 bp DNA Ladder InformationInformation and protocol for NEB's Quick-Load 100 bp DNA Ladder. Note: DO NOT ADD STE or LOADING DYE.
ALU PCR (PV92)
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D1S80 / ALU Protocol - Teacher's GuideTeacher's Guide for D1S80(VNTR) and PV92(ALU) PCR Protocols including master mix amounts and explanation of controls.
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PCR Machine Programing - D1S80/PV92/TAS2R38PCR machine programing temperatures for D1S80 (VNTR), PV92 (Alu), and TAS2R38 (TASTER).
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100 bp DNA Ladder InformationInformation and protocol for NEB's Quick-Load 100 bp DNA Ladder. Note: DO NOT ADD STE or LOADING DYE.
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Resource for Alu (PV92) DescriptionLink for an explanation of the Alu insert polymorphism.