LibGuides: Electron Microscopy Resources & Training: Home

Meet EM Staff

Shared Resources' Cellular Imaging provides transmission and scanning electron microscopy. Services include a variety of sample preparations along with cryo techniques and capabilities.

Resources selected by: Bobbie Schneider, Former Manager
Electron Microscopy Core Facility, Shared Resources, Fred Hutchinson Cancer Research Center

Website: Cellular Imaging

Contact Info: (206) 667-4289 emsr@fredhutch.org

EM Books in Arnold Library

Below are examples of the many books you'll find in the Arnold Library to assist you in your Electron Microscopy work. Browse through additional titles here.

Introduction to Electron Microscopy for Biologists, Volume 88: Methods in Cell Biology by Terry D. Allen Ph.D. D.Sc. (Editor)
Call Number: Shelved under Methods in Cell Biology, v.88
ISBN: 0123743206
This book is ideal for the scientist who may be considering electron microscopy as a tool to extend molecular, biochemical, or light microscope observations to the next level of structural information.
Biological Electron Microscopy: Theory, Techniques, and Troubleshooting by Michael J. Dykstra
Call Number: QH 212 .E4 D996b 2003
ISBN: 0306477491
This text was designed for an introductory one-semester course in biological electron microscopy and is intended to provide an introduction to all of the major technical approaches for sample preparation and instrumentation utilization to answer cytological questions.
Caenorhabditis elegans: Modern Biological Analysis of an Organism, (Methods in Cell Biology, v. 48) by David H. Hall
Call Number: Shelved in the Journal Section under Methods in Cell Biology
See Ch. 17, pages 395-436, "Electron Microscopy and Three-Dimensional Image Reconstruction"

Introduction to Microscopy

Fred Hutch Electron Microscopy Shared Resources - Procedures Manual
Written by experienced EM specialists, this manual covers specific instructions on the use of JEOL 1010 TEM, JEOL 1230 TEM, JEOL JSM-5800 SEM, and the Gatan Digital Camera.
Koehler Illumination
From the Albert Einstein College of Medicine:
The 'what and why Koehler Illumination'. Koehler Illumination is proper alignment of the incident or illuminating light for microscopy. Every time you use the microscope for transmitted light work, whether brightfield, phase or DIC, you must align the condenser lens to assure Koehler illumination is optimal. If you fail to do this, you will have poor resolution, wacky contrast artifacts, and unevenly lit pictures...