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Model Systems in Cancer Research: Mouse

Course describing model organisms and examples of their use in cancer research

Generating a Transgenic Mouse

Generating knock out and knock in mice

Process of Angiogenesis: normal development & cancer

Narrated video has annotated animations and live cell videos.

Critical requirement for Angiogenesis in tumor formation

Online resources

1. Database of mouse models of human cancers. 

2. Information pertaining to research and techniques used for transgenic mice.

3. Online book on mouse genetics by Lee M. Silver.

4. Trans-NIH Knockout Mouse Project (KOMP) - an initiative to generate a public resource of mouse embryonic stem (ES) cells containing a null mutation in every gene in the mouse genome. 

5. Databases of electronic resources.

Lecture Slides and Papers

Mouse (Mus musculus)

Lecture videos are available for viewing in the left column. 

 May 28 class:

The first two videos on the left are part of a single HHMI movie. 

1. Before class - please view video 1. Only minutes 3:00-5:00 about generating a transgenic mouse will be shown in class.

2. During class - video 2 will be used to illustrate key points about generating knock out and knock in mice.

June 4 class:

Videos will help you visualize the process of angiogenesis, please watch before class.

 Required/Encouraged Reading

May 28 class:

1. In this review the Nobel laureate gives an wonderful historical overview of gene targeting in the mouse.

   "Generating mice with targeted mutations" by Mario R. Capecchi

 2. This review provides a comprehensive view of the mouse as a model organism, covering history, techniques and screens.

"The expanding role of mouse genetics for understanding human biology and disease" by Duc Nguyen and Tian Xu

June 4 class:

Articles about the role of angiogenic factors in a mouse models of breast cancer.

1. Deletion of vascular endothelial growth factor in myeloid cells accelerates tumorigenesis by Stockmann C et al.

2. VEGF Restores Delayed Tumor Progression in Tumors Depleted of Macrophages by Lin EY et al.

3. A review providing information that will help you understand details in the above articles.

Monocyte/macrophage infiltration in tumors: modulators of angiogenesis by Dirkx AEM et al.

 Additional Reading

May 28 class:

Review of approaches in mouse genetics - Detailed but easy to understand.

"Tools for targeted manipulation of the mouse genome" by Louise Van Der Weyden et al. 

June 4 class:

1. Recent review discussing targeting of angiogenesis process in cancer.

Molecular Mechanisms and Therapeutic Development of Angiogenesis Inhibitors by Yihai Caoa.

2. General Angiogenesis overview and discussion of its role in cancer and other diseases.

Modulating angiogenesis: more vs less. by Sivakumar B, Harry LE and Paleolog EM.

Books in Library

These books are on course reserve in the first floor copy room of the Arnold Library, Weintraub building for two weeks after class. If you are interested in a more in depth view of the lecture topics take a look.

1. "The laboratory mouse"  by Hedrich, Hans J., Bullock, Gillian R. Very comprehensive book. Includes overview of history, strain development, mouse models of human disease and mouse anatomy.

2. "Transgenic mouse methods and protocols" by Hofker, Marten Harro and  Deursen, Jan van. Part of the Methods in Molecular Biology series, easy to understand, practical information.

3. "Gene Targeting: a practical approach" edited by A.L. Joyner. Compedium of practical protocols and guidance on their use in specific applications. A detailed description of design of gene targeting vectors, ES cell manipulation, producing ES cell chimeras, gene trap approaches. 

4. "Mouse models of human cancer" by Eric C. Holland. Articles by experts describing organ-specific models in clinical, imaging, and molecular terms. Covers the relevant experimental approaches and histological comparisons with human disease. This book is also available as an electronic resource through the Arnold library (click on book title to use the link).